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Chemical Synthesis Of Nucleoside Analogues Pdf Free
55be9034d4 However, the PAN method gave higher and more sustained levels of activity, was easier to handle and incubate, and provided a general batch reactor method for the large scale synthesis of nucleosides such as 2-thio-2'-deoxyuridine. As the synthesis of Y-subs titute d-2,3'-dide oxynucleo sides was not possible by the enzymatic transfer method, an investigation into the conformations adopted by the nucleosides, which were and were not glycosyl donor nucleosides in the transfer reaction, was undertaken using variable temperature 1H NMR. Analysis of the variable temperature 1H NMR data led to information about the conformer populations, the equilibrium constants and, the free energy, enthalpy and entropy of each system. Its ability to act as a glycosyl donor for Ndeoxyribosyltransf erases from lactobacillus was investigated but unfortunately its extreme conformation prevented it from being an active glycosyl donor. Item Type: Thesis or Dissertation (PhD) Subjects: Q Science > QR Microbiology Library of Congress Subject Headings (LCSH): Nucleosides -- Research, Lactic acid bacteria -- Research, Lactobacillus leichmannii -- Research, Enzymes -- Analysis, Enzyme kinetics -- Research Official Date: September 1992 Dates: DateEventSeptember 1992Submitted Institution: University of Warwick Theses Department: Department of Chemistry Thesis Type: PhD Publication Status: Unpublished Supervisor(s)/Advisor: Hutchinson, D. The most successful matrices were octyl Sepharose and Poly(acrylamide-co-N-acryloxysuccinimide) (PAN- 550) gel. The modified nucleoside, ()-l-[(2'P, 4'p)-2"-(hydroxymethyl)-4'- dioxolanyllthymine, [()-Dioxolane-T], in which the Y-carbon atom is replaced by oxygen, was prepared. In this work, a crude extract of N-deoxyribosyltransferase from Lactobacillus leichmannii was used in the chemoenzymatic synthesis of nucleoside analogues with potential antiviral activity, in particular, 9-P-D-2', 3'- dideoxyribofuranosyl 2-aminopurine which had anti-HIV activity.
Little is known about the active sites of the N-deoxyribosyltransferases from lactobacilli, but major factors accounting for this lack of reactivity may be steric hindrance or dipolar effects that inhibit the binding of a substrate to the transferase. It was found that only "'flexible" sugar rings without extremes of conformation were active as glycosyl donors in our system. Downloads per month over past year View more statistics .. Hicks, Nicola (1992) The enzymatic and chemical synthesis of nucleoside analogues using N-deoxyribosyltransferase from Lactobacillus leichmannii. Abstract. Request changes or add full text files to a record . PhD thesis, University of Warwick. (David Wesley) Sponsors: Science and Engineering Research Council (Great Britain) (SERC) Format of File: pdf Extent: 260 leaves : ill., charts Language: eng URI: .